DNA probes using fluorescence resonance energy transfer (FRET): designs and applications.
نویسنده
چکیده
Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET The approaches used in the design of new DNA FRET probes are discussed.
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عنوان ژورنال:
- BioTechniques
دوره 31 5 شماره
صفحات -
تاریخ انتشار 2001